Enhanced Bio-Barcode Immunoassay Using Droplet Digital PCR for Multiplex Detection of Organophosphate Pesticides

A bio-barcode immunoassay based on droplet digital polymerase chain reaction (ddPCR) was developed to simultaneously quantify triazophos, parathion, and chlorpyrifos in apple, cucumber, cabbage, pear. Three gold nanoparticle (AuNP) probes magnetic (MNP) were prepared, binding through their antibodies with the three pesticides same tube. groups of primers, probes, templates, designed ensure specificity method. Under optimal conditions, detection limits (expressed as IC10) 0.22, 0.45, 4.49 ng mL-1, respectively. The linear ranges 0.01-20, 0.1-100, 0.1-500 correlation coefficients (R2) 0.9661, 0.9834, 0.9612, recoveries relative standard deviations (RSDs) 75.5-98.9 8.3-16.7%. This study provides first insights into ddPCR for determination organophosphate pesticides. It also laid foundation high-throughput other small molecules.